Temporal changes in vascular angiotensin receptors in female control and protein-restricted offspring. Real-time PCR was used to assess vascular AT1aR mRNA expression at 1 month of age (A), 3 months of age (B), and 6 months of age (C) and vascular AT2R mRNA expression of in 1 month of age (D), 3 months of age (E), and 6 months of age (F). Quantitation of vascular RAS components was normalized relative to 18S levels. The ratio of AT1aR/AT2R at 1 month of age (G), 3 months of age (H), and 6 months of age (I) is presented. n=5–8 in each group.*P < 0.05 vs. control.