Figure 1. IgM and IgG1 transcript expression levels in resting and stimulated splenic B cells.
Expression levels for (A) μ germline transcripts (μGLT), (B) γ1 germline transcripts (γ1GLT), (C) γ1 post switched transcripts (γ1PST), and (D) β-actin were measured by quantitative real time RT- PCR using cDNAs from splenic B cells of C57BL/6 wild type (solid circles), SμTR−/− (open circles), and AID−/− (solid squares) mice. Splenic B cells were stimulated with LPS and IL4 over a 48 hour time course (0hr, 12hr, 24hr, 36, and 48hr). PST samples included a 96 hour time point. Results were normalized to Igβ expression. Real time PCR was performed in triplicate. Error bars represent standard estimated mean (SEM) from three independent experiments.