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The functionality and specificity of anti-CEA and anti-NIP scFv type XV trimerbodies was demonstrated by western blot (A), and by ELISA (B and C) against plastic immobilized BSA, NIP10-BSA conjugates and CEA. MFE23 anti-CEA and B1.8 anti-NIP scFvs were used as controls. Antigen titration ELISA of purified B1.8 scFv and B1.8 scFv type XV trimerbody against plastic immobilized NIP10-BSA conjugates (C).
