Table 5.

State of the art antibody production methods and their dependence on Cabilly II and III

Production method	Company		Patent	Protected by Cabilly II?	Protected by Cabilly III?
Heterologous Dimeric Protein Production in Heterokaryons	Neugenesis		US5643745	No, because two cell lines are independently transformed with HC DNA and LC DNA, and are then fused	Probably yes
Milk secretion in transgenic mammals	GTC Biotherapeutics		US5827690	No, because transgenic animal comprises a full repertoire of heavy and LC genes, i.e. no transformation of a host cell, and expression takes place in hybridoma cells	No, because no recombinant host cells (cells which have been transformed with vectors used)
Hybridoma cells obtained from transgenic rodents or mice	Regeneron VelocImmune		US7105348
Expression of monoclonal antibodies in ciliate host cells with different mating types	Cilian AG		WO2011107520	No, because two cells which have been trasformed with HC and Lc, respectively, can be conjugated to express full antibody	Probably yes