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. Author manuscript; available in PMC: 2013 May 24.
Published in final edited form as: Neuron. 2012 May 24;74(4):676–690. doi: 10.1016/j.neuron.2012.03.025

Figure 2. Sema6D is inhibitory to outgrowth of crossed RGCs in vitro.

Figure 2

(A) A Sema6D function–blocking antibody (αSema6D) specifically reduces RGC neurite outgrowth from DT explants, but has no effect on VT explants on WT chiasm cells. (B) Summary of results in Figure 2A. Note that blocking Sema6D functions leads to less supportive to outgrowth of crossed RGC axons. (C) Representative E14.5 DT explants plated on HEK cells expressing an empty vector (Ctr) or Sema6D. (D) DT neurite outgrowth is reduced on Sema6D+ HEK cells, but this growth reduction is not observed in the presence of αSema6D. VT outgrowth is not changed from control levels in explants growth on Sema6D+ HEK cells, with or without αSema6D. (E) Summary of results in Figure 2D. Note that Sema6D+ HEK cells are inhibitory to neurite outgrowth from crossed RGC axons. Scale bars: 200µm, (n) = number of explants for each condition, * p<0.01.