Skip to main content
. Author manuscript; available in PMC: 2013 May 24.
Published in final edited form as: Neuron. 2012 May 24;74(4):676–690. doi: 10.1016/j.neuron.2012.03.025

Figure 6. Nr-CAM is a receptor for Sema6D.

Figure 6

(A) Expression of Plexin-A1, Neuropilin1, Nr-CAM, Neurofascin, TAG-1 and L1 are more highly expressed on DT axons and growth cones compared to VT. (B) AP-Sema6D binds to HEK cells transfected with Plexin-A1 or Nr-CAM, but not Neuropilin1 and other CAMs. (C) AP-Sema6D binding to retinal axons (red, neurofilament (NF)) is reduced in Nr-CAM−/− and Plexin-A1−/− mutant brain sections through the optic chiasm compared to WT and is completely absent in Plexin-A1−/−;Nr-CAM−/− double mutant brains. (D) Immunoprecipitation assay demonstrating binding of Nr-CAM, but not Neurofascin 186 (Nfasc) to Plexin-A1. (E) Immunoprecipitation assay showing Nr-CAM and Sema6D binding. Scale bars, 5µm in A; 20µm in B; 200µm in C.