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. Author manuscript; available in PMC: 2013 May 25.
Published in final edited form as: Cell. 2012 May 25;149(5):1125–1139. doi: 10.1016/j.cell.2012.03.039

Figure 1. AnkG clustering follows and is dispensible for axon specification.

Figure 1

(A–J) Neurons electroporated in utero at E14 with GFP plasmids and immunostained for GFP (green) and ankG (red) at embryonic day 16 (E16) (A, B), E18 (C, D), postnatal day 1 (P1) (E, F), P5 (G, H), and P28 (I, J). Scale bars: A, 25 μm; B, D, F, J, 10 μm; C, E, G, I, 50 μm; H, 5 μm.

(K) The percentage of GFP-electroporated neurons with an AIS defined by ankG (vertical bars) and their positions in the cortical plate (dots). The number of cells analyzed is indicated in parentheses.

(L) The percentage of GFP-electroporated neurons with basal dendrites.

(M) Cartoon illustrating the transition in cellular morphology, the position and length of ankG along the axon.

(N) The length and distance from the cell body of the ankG-labeled AIS.

(O) GFP-labeled axons from neurons expressing the ankG-shRNA construct cross the corpus callosum. Scale bar, 50 μm.

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