Skip to main content
. Author manuscript; available in PMC: 2013 May 25.
Published in final edited form as: Cell. 2012 May 25;149(5):1125–1139. doi: 10.1016/j.cell.2012.03.039

Figure 3. Developmental assembly of the distal axonal cytoskeleton.

Figure 3

(A) Stage 3 hippocampal neuron labeled for MAP2 (green) and αII spectrin (red). The arrowhead indicates the growth cone. Scale bar, 10 μm.

(B) The ratio of growth cone to dendrite fluorescence intensity for ankB, αII spectrin, and βII spectrin immunofluorescence (blue squares).

(C–E) Cultured hippocampal neurons transfected with 220 kD ankB-GFP (MBSBDCB), or chimeras of ankB-GFP and ankG-GFP, and labeled for GFP, βIV spectrin and MAP2. The analysis was performed at 7 DIV. Scale bar, 25 μm.

(F) Cultured hippocampal neuron transfected with A1000P ankB-GFP, and labeled for GFP, βIV spectrin, and MAP2. The analysis was performed at 7 DIV. Scale bar, 25 μm.

(G) Quantification of axonal polarity using ankB and ankG chimeras. The number of cells analyzed is shown in parentheses.

(H, I) Stage 3 hippocampal neuron labeled for ankB (green) and KAP3 (H; red) or KIF3 (I; red). The arrowhead indicates the growth cone. Scale bar, 10 μm.

(J) Immunoprecipitation reactions from brain lysates using antibodies against αII spectrin, ankB, KIF3, and KAP3.

HHS Vulnerability Disclosure