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. Author manuscript; available in PMC: 2013 May 2.
Published in final edited form as: Cell Metab. 2012 May 2;15(5):691–702. doi: 10.1016/j.cmet.2012.04.008

Figure 5. TG Hydrolase Activity of ADPN.

Figure 5

(A) TG hydrolase activity assay using radiolabeled triolein as substrate in the presence of 5 μg purified TF, mAdpn, mI148M, hADPN, hI48M, and (B) bacterial total lysates (40 μg lysate protein) overexpressing TF, mAdpn, mI148M, hADPN, hI148M, and strep-tagged mATGL. Assays were performed in the absence or presence of purified mCGI-58. Data are shown as mean ± SD and represent three independent experiments. Statistical significance was determined by a two-tailed Student’s t test, *p < 0.05, ***p < 0.001. FFA, free fatty acid.