Figure 8.

AFSCs viability comparing different cryoprotectants (Me₂SO, glycerol, sucrose, and trehalose) and programmed and nonprogrammed freezing protocols after 3 and 6 months. (a) viability in nonprogrammed method for 3 months; (b) viability in programmed method for 3 months; (c) viability in nonprogrammed method for 6 months; (d) viability in programmed method for 6 months. 10% Me₂SO showed the higher viability rate similar to NR control in all cases. NR-AFSCs viability control measured before freezing procedure. M-Me₂SO, G-glycerol, S-sucrose, and T-trehalose. Means were compared by one-way ANOVA Dunnett's (***P < 0.05) and Bonferroni (^#P < 0,05) post tests.