Table 3.
After programmed freezing, AFSCs (n = 5) maintained their specific mesenchymal membrane proteins evaluated by flow cytometry (CD29, CD44, and CD105 positive; CD14, CD34, and CD45 negative). The number of acquired events was 15,000 in each acquisition. Results are shown as percentage ±SEM.
| Programmed | CD14 PE | CD34 FITC | CD45 FITC | CD29 PE | CD44 FITC | CD105 PE |
|---|---|---|---|---|---|---|
| Me2SO 5% | 1.88 ± 0.35 | 0.58 ± 0.22 | 0.85 ± 0.53 | 96.06 ± 2.61 | 97.03 ± 2.06 | 98.82 ± 1.06 |
| Me2SO 10% | 0.92 ± 0.46 | 0.75 ± 0.13 | 0.76 ± 0.44 | 99.67 ± 0.16 | 99.88 ± 0.10 | 99.02 ± 0.15 |
| Glycerol 5% | 0.91 ± 0.74 | 0.18 ± 0.08 | 0.39 ± 0.29 | 99.48 ± 0.40 | 99.85 ± 0.16 | 99.51 ± 0.17 |
| Glycerol 10% | 1.76 ± 0.96 | 1.07 ± 0.97 | 1.12 ± 1.01 | 99.71 ± 0.16 | 99.87 ± 0.08 | 99.11 ± 0.39 |
| Sucrose 30 mM | 0.62 ± 0.48 | 0.22 ± 0.14 | 0.48 ± 0.15 | 99.29 ± 0.19 | 99.95 ± 0.03 | 99.65 ± 0.06 |
| Sucrose 60 mM | 1.36 ± 0.08 | 0.96 ± 0.06 | 1.51 ± 0.83 | 99.35 ± 0.28 | 99.17 ± 0.66 | 99.02 ± 0.20 |
| Trehalose 60 mM | 1.25 ± 0.41 | 0.31 ± 0.25 | 0.48 ± 0.25 | 97.77 ± 1.31 | 99.85 ± 0.11 | 98.90 ± 0.66 |
| Trehalose 100 mM | 0.53 ± 0.88 | 0.46 ± 0.36 | 0.60 ± 0.56 | 99.50 ± 0.36 | 99.83 ± 0.18 | 98.94 ± 0.76 |