Table 1.
Lysozyme and Cu2+ binding capacities of nylon membranes modified with different films.a
| Polyelectrolyte films in nylon membrane | pH of PAH and/or PAA deposition solutions | Lysozyme binding from breakthrough curves (mg/cm3) | Lysozyme binding From elution (mg/cm3) | Cu2+ binding (mg/cm3)c |
|---|---|---|---|---|
| PSS/PAH/PAA | 2 | 90±1 | 87±1 | 15±1 |
| 3 | 106±2 | 106±6 | 11±2 | |
| 4 | 49±2 | 49±1 | 6±1 | |
| 5 | 37±4 | 33±3 | 3±2 | |
| PAA | 2 | 78±1 | 77±1 | 12+2 |
| 3 | 89±4 | 89±2 | 13±2 | |
| 4 | 22±9 | 22±6 | 4±2 | |
| 5 | 14±6 | 14±2 | 2±1 | |
| PAA/PAH/PAA | 3 | 107±2 | 115±2 | 14±1 |
| PAA/PEI/PAA | 3 | 120±6 | 130±4 | 18±2 |
| PAA/PEI/PAAb | 3 | 101±5 | 112±4 | - |
a
Each experiment was performed with two different membranes, and the ± values represent the difference between the average and the data points.
b
Lysozyme flow rate of 30 mL/min. In all other cases the lysozyme flow rate was 1 mL/min.
c
Binding capacity after derivatization with aminobutyl NTA.