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. 2012 Feb 15;302(9):C1405–C1412. doi: 10.1152/ajpcell.00435.2011

Table 1.

Summary of MTX and PMX influx kinetic values for the wild-type and P425R mutant PCFT

Vmax
Kt, μM Mutant/WT Ki, μM Rate: pmol·mg protein−1·min−1 Relative expression at the cell surface (P425R/WT) Corrected for expression Mutant/WT–corrected for expression
WT
    MTX 2.03 ± 0.54 3.1 ± 0.4 277.4 ± 22.9 1.0
    PMX 0.60 ± 0.08 102.9 ± 3.9
P425R
    MTX 10.1 ± 1.53 4.98 29 ± 2 120.4 ± 8.6 0.58 208 0.73
    PMX 0.41 ± 0.07 0.68 25.3 ± 1.2 44 0.44

Values are means ± SE. A comparison of methotrexate (MTX) and pemetrexed (PMX) influx kinetics (Kt and Kt) based on experiments represented in the data of Figs. 4 and 5. Relative expression was measured by ImagejJ densitometry from 2 separate experiments and used to normalize the influx Vmax values. PCFT, proton-coupled folate transporter; WT, wild type.