Table 1.

Primer sequences

Target	Forward primer sequence (5′-3′)	Reverse primer sequence (5′-3′)	Annealing temperature (°C)
Canine cardiac actin gene	AGCACTGTTAGAGACACCTG	CGGATAGCACGTTGTTGGCA	57
Canine adenovirus-1	TGCTGCCACAATGGTCTTAC	CCACAGTGGGGTTTCTGAAC	60
Canine parvovirus	GACTGGGAATCGGAAGTTGA	CAATGCCAGCCTTGATCTTT	59
Canine herpesvirus	CTCTGGGAGATGCGATTGAT	AAACTTCAGGAGCGCTTTCA	59
Pathogenic Leptospira species	TGAGTAACACGTGGGTAATCTTCC	CAGGTACCATCATCACATYGCTGC	62