Fig. 5.

The effect of pomegranate juice on the syncytiotrophoblasts exposed to cobalt chloride or staurosporine. A: diagram of pomegranate juice, cobalt chloride, or staurosporine exposure for the primary human trophoblasts. The trophoblasts were pretreated with pomegranate juice (1%, vol/vol) or glucose (7.5 mM) for 24 h before exposure to cobalt chloride for up to 24 h, or staurosporine for up to 4 h. B: top, Western blot of cl-Cyt 18 in syncytiotrophoblasts cultured in regular DMEM for 52 h were exposed to cobalt chloride (CoCl₂, 200 nM) for up to 24 h. Bottom, summary graphs of Western blots of cl-Cyt 18 at time (t) = 24 h, n = 3. *P < 0.05, t-test. C: top, Western blots of cl-Parp and cl-Cyt 18 in syncytiotrophoblasts exposed to CoCl₂ with pomegranate juice or glucose, as control, for up to 24 h. Middle and bottom, summary graphs of densitometry of cl-Parp and cl-Cyt 18 expression. D: LDH analysis of syncytiotrophoblasts exposed to CoCl₂ with pomegranate compared with control. E: top, Western blots of cl-Parp and cl-Cyt 18 in staurosporine (0.5 μM)-exposed syncytiotrophoblasts cultured in regular DMEM for up to 4 h. Middle and bottom, summary graphs of Western blots of cl-Parp and cl-Cyt 18, n = 3. *P < 0.05, 1-way ANOVA, with Bonferroni post hoc test. F: top, Western blots of cl-Parp and cl-Cyt 18 in syncytiotrophoblasts exposed to staurosporine compared with control for up to 4 h with pomegranate juice compared with control. Middle and bottom, summary graphs of densitometry of cl-Parp and cl-Cyt 18. G: LDH analysis of syncytiotrophoblasts exposed to staurosporine with pomegranate juice compared with control. In C, D, F, and G, n = 3. *P < 0.05, 2-way ANOVA, with Bonferroni post hoc test.