Fig. 3.

Phosphorylation of TBC1D1 in TA muscles from WT and TBC1D1 deletion mutant mice upon in situ contraction. Hindlimb muscles were contracted in situ via sciatic nerve stimulation for 10 min on one leg (Ctxn) from anesthetized TBC1D1 deletion mutant mice and WT littermates (8 wk old), and the other leg served as sham-operated control (basal). Total and phosphorylated TBC1D1 and AMPK were determined in 40 μg of muscle extracts using anti-TBC1D1, anti-AMPKα2, and anti-pT172AMPK antibodies, respectively. PAS signal at ∼150–160 kDa was detected in muscle extract using PAS antibody.