Protein kinase B (Akt), mammalian target of rapamycin (mTOR), and downstream signaling. Data represent phosphorylation of Akt at Ser473 (A), mTOR at Ser2448 (B), S6 kinase 1 (S6K1) at Thr389 (C), S6K1 at Thr421/Ser424 (D), 4E binding protein 1 (4EBP1) at Thr37/46 (E), and ribosomal protein S6 (rpS6) at Ser240/244 (F) in the postabsorptive state (basal) and 1 and 3 h after ingestion of EAA in skeletal muscle of older adults (n = 6). *Different from basal (P < 0.05). #Different from postbed rest at corresponding time point (P < 0.05). Values are presented as means ± SE.