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Expression and phosphorylation of tight junction proteins in hBMECs following 6 h of IL-1β treatment. Western blot data shows no significant (P > 0.05) change in expression of claudin-5 or zona occludens (ZO)-1 in hBMEC whole cell lysates (A) or membrane fractions (B). Representative Western blots are shown (top) together with quantified data normalized to β-actin (bottom) from n ≥ 4 independent experiments.
