Fig. 4.
Constitutively active Lck (LckY505) reduces the effect of PKAI on KV1.3 channels. KV1.3 currents were recorded in Jurkat cell after a 24-h transfection with either LckY505F or the empty vector (control). Representative currents before (black) and after 8-BrcAMP application (1 mM, gray) are shown at left. Currents were elicited by depolarizing pulses to +50 mV every 30 s (HP = −80 mV). Average inhibition of KV1.3 current is shown in at right for LckY505F (gray; n = 5)- and empty vector-transfected Jurkat cells (control, black, n = 6).