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Purification of A. baumannii EF-Tu. Purification of A. baumannii rEF-Tu. (a) Overexpressed (lane 1) and column-purified rEF-Tu (lane 2). (b) Immunoblot of column-purified rEF-Tu with anti-His-tag monoclonal antibody. (c) Immunoblot of A. baumannii cell lysate with rabbit prebleed (lane 1) and anti-rEF-Tu antibodies (2). Immunoblot of rEF-Tu with the anti-rEF-Tu (3).
