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. 2012 May 15;2012:128705. doi: 10.1100/2012/128705

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Copyright © 2012 Shatha F. Dallo et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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EF-Tu detected in OMV and OM fractions. Panel (A): (a) SDS-PAGE of proteins from OM (lane 1) and OMV fractions (2). (b) Western blot of proteins from OM (lane 1) and OMV fractions (2) reacted with the EF-Tu antibody diluted at 1 : 3000. Control blot with pre-immune serum (lane 3). Panel (B): 2D-based Western blot. Proteins from OM (a and c) and OMV fractions (b and d) were resolved by isoelectric focusing (1st D) and then separated on a second dimension SDS-PAGE (2nd D). Proteins from the gel were blotted onto PVDF membranes and probed with the anti-EF-Tu antibodies (c and d). Arrows: EF-Tu. Blot with pre-immune serum not shown.