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Binding of EF-Tu to fibronectin examined by Western-blot-based binding assays. (a) Proteins were resolved by SDS-PAGE and blotted onto PVDF membranes. Proteins from OM (lane 1) and OMVs (2) and the purified rEF-Tu (3) were probed with FN and anti-FN. rEF-Tu (4) and proteins from OM (not shown) probed with anti-FN alone. (b) Immunoblot of 2-DE of proteins from OM probed with FN followed by anti-FN. Arrow indicates EF-Tu confirmed by protein sequencing.
