Figure 1.

Aβ stain by immunoelectron microscopy. 36 hours after the intracerebral injection of Aβ tissues from the injected area were obtained and subjected to immunohistochemistry by using a primary polyclonal antibody against Aβ. Deposits of Aβ forming deposits in the extracellular space were revealed by conventional light microscopy (data not shown). Aβ immunoreactivity was then revealed with a 6 nm gold label and observed in a transmission electron microscope which allows us to identify (a) deposits of Aβ within myelin axons (black arrows) and in the vasculature (white arrows). (b) Deposits of Aβ (black arrows) penetrate the axon membranes causing demyelination and appear in the axons. Axons look like bulb onions. (c) Aβ appears within the mitochondria finally, where it forms deposits along the cristae (black arrows) and causes intense inflammation, destruction of membranes, and vacuolization (magnification at 27800x).