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Effect of increased media osmolarity, Na, or chloride concentration on flow-stimulated endothelin (ET)-1 mRNA content in mpkCCDc14 cells. Each data point represents the comparison of ET-1 mRNA in cells exposed to flow vs. cells not undergoing flow, with both flowed and stationary cells exposed to identical media osmolarity and electrolyte concentrations; n = 12 each data point. *P < 0.05 vs. cells not exposed to flow. **P < 0.05 vs. cells exposed to 300 mosmol/l NaCl.
