Fig. 4.

Effect of aldosterone ± amiloride on flow-stimulated ET-1 mRNA content in mpkCCDc14 cells. Cells were pretreated with 1 μM aldosterone for 2 days and pretreated with 1 μM amiloride for 30 min. Each data point represents the comparison of ET-1 mRNA in cells exposed to flow vs. cells not undergoing flow, with both flowed and stationary cells exposed to identical media and reagents. All studies were done in HBSS (300 mosmol/l); n = 12 each data point. *P < 0.05 vs. cells not exposed to flow. **P < 0.05 vs. aldosterone alone.