Fig. 5.

Effect of plasma membrane Na calcium exchanger inhibition on flow-stimulated ET-1 mRNA content in mpkCCDc14 cells under static (no flow) and flow conditions. Cells pretreated for 30 min with 3 μM SEA0400 (n = 7) or 10 μM KB-R7943 (n = 12). Each data point represents the comparison of ET-1 mRNA in cells exposed to flow vs. cells not undergoing flow, with both flowed and stationary cells exposed to identical media and reagents. All studies were done in HBSS (300 mosmol/l). *P < 0.05 vs. cells not exposed to flow.