Fig. 4.

JNK mediates IFNγ-induced inhibition of ppET-1 promoter activity. A: HSCs were cotransfected with ppET-1 promoter luciferase reporter plasmid (ppET1–2121+131) with expression plasmid containing dominant-negative JNK1/2 (JNK1/2-DN) or dominant-active JNK1/2 (JNK1/2-KM) or control expression vector for overnight, exposed to IFNγ (500 IU/ml) for 2 days. The luciferase activity was assayed (n = 3; *P < 0.05 vs. no IFNγ; #P < 0.05 vs. control). B: cotransfection was performed as the same as in A except ppET1–2121+131 construct was replaced with the ppET1–2121+131 containing AP-1 site mutation (n = 3; *P < 0.05 vs. no IFNγ).