Fig. 1.

Ligand-induced chromatin recruitment of Set7/9 to the promoters of FXR-target genes and the associated histone H3 activation mark. Chromatin immunoprecipitation (ChIP) analysis of hepatoma cells (Huh-7) showed recruitment of FXR, Set7/9, CARM1, and the H3K4 monomethylation mark of histone H3 to the endogenous short heterodimer partner (SHP; A) and human bile salt export pump (BSEP; B) promoters in the presence of the farnesoid X receptor (FXR)-ligand (GW4064). Schematic representation of the primers used for ChIP and the location of the sites on the SHP and BSEP promoters are shown below representative gels.