Fig. 2.

The effect of short interfering RNAs (siRNA) knockdown of Set7/9 expression on ligand-induced occupancy of Set7/9 and FXR and on H3K4 monomethylation at the endogenous BSEP and SHP promoters. siRNAs were used to reduce cellular Set7/9 levels with a nonsilencing siRNA used as control. Treatment of Huh-7 cells with siRNA against Set7/9 markedly reduced ligand-induced occupancy of Set7/9 and FXR at the SHP (A) and BSEP (B) promoters and decreased the H3K4 methylation mark, as assessed by real-time quantitative PCR. Each independent ChIP data point is the average of normalized values from PCR runs in duplicate wells. Each histogram is the average mean ± SE from independent ChIP experiments of n = 3. *P < 0.05, #P < 0.001.