Fig. 3.

Enzymes that regulate bile acid synthesis are downregulated and farnesoid X receptor (Fxr) and small heterodimer partner (Shp) are upregulated in Nrf2^−/− mice. A: relative mRNA expression of genes involved in bile acid synthesis [cytochrome P-450 (Cyp7a1, Cyp8b1, and Cyp27a1)] in liver of wild-type and Nrf2^−/− mice. B: mRNA expression of the nuclear receptor Fxr and Shp in liver of wild-type and Nrf2^−/− mice. Data are expressed as fold change relative to wild-type mice. Values are means ± SD of 5–6 animals. Amount of mRNA from wild-type mice is set as 1. **P < 0.01. ***P < 0.001.