Table 1.
Mouse primers/probes used in TaqMan real-time PCR
| Gene | Gene Assay ID No. | Primer Sequences (5′ to 3′) | GenBank Accession No. |
|---|---|---|---|
| Cyp7a1 | Mm00484152_m1 | ||
| Cyp8b1 | Mm00501637_s1 | ||
| Cyp27a1 | Mm00470430_m1 | ||
| Ntcp | Mm00441421_m1 | ||
| Oatp1a1 | Mm00649796_m1 | ||
| Bsep | Mm00445168_m1 | ||
| Mdr1a | Mm00440761_m1 | ||
| Mdr1b | Mm00440736_m1 | ||
| Mrp2 | Mm00496899_m1 | ||
| Mrp3 | Mm00551550_m1 | ||
| Mrp4 | Forward: CATCAAGTCCAGGGAAAAGGTTG | AK052778 | |
| Reverse: GAGGGCCGAGATGAGGGAG | |||
| Probe: TGGGCAGAACCGGAGCTGGGAAA | |||
| Ostα | Forward: TGTTCCAGGTGCTTGTCATCC | NM_145932 | |
| Reverse: CCACTGTTAGCCAAGATGGAGAA | |||
| Probe: CCGCCCTGCAGCCTGCCAT | |||
| Ostβ | Forward: ATGCGGCTCCTTGGAATTA | NM_178933 | |
| Reverse: GGAGGAACATGCTTGTCATGAC | |||
| Probe: TCCATCCTGGTCCTGGCAGTCCTG | |||
| Asbt | Mm00488258_m1 | ||
| Fxr | Mm00436419_m1 | ||
| Shp | Mm00442278_m1 | ||
| Fgf15 | Mm00433278_m1 | ||
| Pxr | Mm00803095_m1 | ||
| Car | Mm00437986_m1 | ||
| Cyp3a11 | Mm00731567_m1 | ||
| Sult2a1 | Forward: GTCACTCGGAACTTATTTTGAATGGT | ||
| Reverse: AGCCAGCCACGAACATGCT | |||
| Probe: CCTCAAAGGAAATGTTCTATTCGG | |||
| Ugt1a1 | Forward: GAGGCTTTGGGCAGAATTCC | ||
| Reverse: TTTGCAAGATTCGATGGTCTAGTTC | |||
| Probe: CAGACGGTCCTGTGGCGCTACACC | |||
| Procollagen1α(I) | Mm00801666_m1 | ||
| Gapdh | Forward: GCCCAGAACATCATCCCTGC | NM_001001978 | |
| Reverse: CCGTTCAGCTCTGGGATGACC | |||
| Probe: TCCACTGGTGCTGCCAAGGCTGTG |
Except for mouse Gapdh, multidrug resistance-associated protein (Mrp4), organic solute transporters (Ostα and Ostβ), sulfotransferase (Sult2a1), and UDP-glucuronosyltransferase (Ugt1a1), which are designed according to the mouse sequence using Primer Express software (Applied Biosystems), all primers are proprietary to Applied Biosystems and labeled with 6-carboxyfluorescein (FAM). A labeled probe for Gapdh is 2,7-dimethoxy-4,5-dichloro-6-carboxyfluorescein (JOE), and labeled probes for Mrp4, Ostα, Ostβ, Sult2a1, and Ugt1a1 are FAM. Cyp, cytochrome P-450; Ntcp, sodium taurocholate-cotransporting polypeptide; Oatp, organic anion-transporting polypeptide; Bsep, bile salt export pump; Mdr, multidrug resistance; Asbt, apical sodium-dependent bile acid transporter; Fxr, farnesoid X receptor; Shp, small heterodimer partner; Pxr, pregnane X receptor; Car, constitutive androstane receptor.