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Knockdown of K-ras blocks activation of ERK. Two different cell clones stably transfected with a small interfering (siRNA) against mutant K-rasV12 in SW480 colon cancer cells (SW480siKrasV12) or stably transfected with a control siRNA (SW480sicontrol) were analyzed for K-ras expression by Western blotting. Silencing of K-ras disrupts activation of ERK 1 and 2, shown as a reduction in phosphorylation (p-ERK1 and p-ERK2).
