Fig. 1.

Postsort analysis of the different fluorescence-activated cell sorting (FACS) isolated Sox9-EGFP cell populations. A: representative photographs of the 4 Sox9-EGFP cell fractions isolated by FACS demonstrate that they express appropriate intensities of enhanced green fluorescence protein (EGFP) fluorescence (magnification ×40). B: data from flow cytometry used to assess the postsort efficiencies and potential cross-contamination of cells that do not express Sox9-EGFP and those expressing sublow, low, and high levels of Sox9-EGFP (Sox9-EGFP Negative, Sublow, Low, and High cells, respectively) with other Sox9-EGFP cell populations. Data are expressed as means and SE of the postsort percentages of corresponding cells collected for each sort (n ≥ 9 in each group). Note that each sorted cell population contains more than 70% of the appropriate cell type and presents minimal contamination with other cells. Importantly, the postsort efficiencies and cross-contamination values do not significantly differ in cells from nonirradiated and irradiated mice.