Table 1.
Genes upregulated specifically in Sox9-EGFP Low cells
| Symbol | Gene Name | Fold Change | ID |
|---|---|---|---|
| LGR5 | leucine-rich repeat containing G protein-coupled receptor 5 | 11.72 | 14160 |
| SCN2B | sodium channel, voltage-gated, type II, beta | 10.44 | 72821 |
| TNFRSF19 | tumor necrosis factor receptor superfamily, member 19 | 10.08 | 29820 |
| NKD1 | naked cuticle homolog 1 (Drosophila) | 8.43 | 93960 |
| SLCO3A1 | solute carrier organic anion transporter family, member 3A1 | 7.01 | 108116 |
| ASCL2 | achaete-scute complex homolog 2 (Drosophila) | 6.86 | 17173 |
| PHTPRO | protein tyrosine phosphatase, receptor type, O | 6.73 | 19277 |
| RGMB | RGM domain family, member B | 6.55 | 68799 |
| AQP4 | aquaporin 4 | 5.63 | 11829 |
| CDC42EP1 | CDC42 effector protein (Rho GTPase binding) 1 | 5.27 | 104445 |
| CRLF1 | cytokine receptor-like factor 1 | 4.56 | 12931 |
| ZBTB12 | zinc finger and BTB domain containing 12 | 3.45 | 193736 |
| CITED4 | Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 4 | 2.76 | 56222 |
| FGFRL1 | fibroblast growth factor receptor-like 1 | 2.62 | 116701 |
| RNF43 | ring finger protein 43 | 2.50 | 207742 |
| SLC23A3 | solute carrier family 23 (nucleobase transporters), member 3 | 2.42 | 22626 |
| LAMB3 | laminin, beta 3 | 2.26 | 16780 |
| ETS2 | v-ets erythroblastosis virus E26 oncogene homolog 2 (avian) | 2.03 | 23872 |
| TNS3 | tensin 3 | 1.86 | 319939 |
| SP5 | Sp5 transcription factor | 1.85 | 64406 |
| PDGFA | platelet-derived growth factor alpha polypeptide | 1.44 | 18590 |
Genes regulated specifically and significantly in the indicated Sox9-EGFP cell population vs. all other fluorescence-activated cell sorting (FACS)-isolated cell populations (n = 7; P < 0.05). Twenty-one genes present in the list of 164 genes specifically upregulated in cells expressing low levels of Sox9-EGFP (Sox9-EGFP Low cells) have previously been reported as enriched in intestinal stem cells (ISCs) expressing high levels of Lgr5-EGFP (Lgr5-EGFPhi) (65). Lgr5 and Ascl2 (bold font) are well-accepted valid ISC markers (3, 65). Fold change is expressed vs. gene expression in cells that do not express Sox9-EGFP (Sox9-EGFP Negative cells). ID, Entrez Gene identification number.