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Effect of iron feeding on PAP7 localization in the rat duodenum. As described in the materials and methods immunofluorescence staining of PAP7 and divalent metal transporter 1 (DMT1) was conducted on sections of the rat duodenum before (top) and at 1 h (bottom) after iron feeding. Simultaneously, the nuclei were stained with TOPRO-3. Arrows indicate the brush border membrane (BBM) and basolateral membrane (BLM).
