Table 1.
Effect of downregulation of PAP7 by siRNA on cell growth
| Days After Transfection |
|||||
|---|---|---|---|---|---|
| Transfection | 2 | 3 | 4 | 5 | 6 |
| Mock | 1.3 ± 0.1 | 3.1 ± 0.2 | 6.9 ± 0.5 | 12.3 ± 2.0 | 15.1 ± 2.3 |
| siPAP7-3 | 1.2 ± 0.2 | 1.9 ± 0.3* | 3.8 ± 0.3* | 8.0 ± 0.5* | 14.8 ± 2.2 |
| (P = 0.0006) | (P = 0.003) | (P = 0.02) | |||
| siPAP7-8 | 1.3 ± 0.1 | 2.8 ± 0.4 | 7.1 ± 0.9 | 11.7 ± 1.5 | 17.1 ± 1.9 |
| siPAP7-10 | 1.2 ± 0.2 | 2.2 ± 0.5* | 4.5 ± 0.9* | 9.1 ± 0.8 | 17.3 ± 1.0 |
| (P = 0.001) | (P = 0.0006) | ||||
As detailed in materials and methods, K562 cell numbers were determined at days 2–6 following transfection. Shown are the cell numbers ×105/ml (means ± SD) for 4 experiments for either mock-transfected cells or cells transfected with small interfering (si) peripheral benzodiazepine receptor-associated protein 7 (PAP7)- 3, -8, or -10.
*
Significant difference from mock-transfected cells on the same day with the P value determined by an unpaired t-test shown in parentheses.