Table 1.
Summary of colorectal afferent activation and sensitization in mouse and rat in vitro organ-nerve preparations (afferent class)
| Mucosal | Muscular | Muscular-Mucosal | Serosal | Mesenteric | MIAs | Ref. No. | |
|---|---|---|---|---|---|---|---|
| Agonists/Mediators | |||||||
| 48/80 | U (r-LSN) | U (r-LSN) | (21) | ||||
| 5-HT | A (r-LSN) | A (r-LSN) | 21) | ||||
| AHS | ↓(m-PN) | ↓(m-PN) | A (m-PN) | AS (m-PN) | (55,56) | ||
| ATP | A (m-LSN, PN) | A (m-LSN) | (12) | ||||
| U (m-PN) | |||||||
| Bile | A (r-LSN) | U (r-LSN) | A (r-LSN) | (63) | |||
| U (m-LSN, PN) | (32) | ||||||
| BK | AS (m-LSN) | A (m-LSN) | (15) | ||||
| A (m-PN) | U (m-PN) | ||||||
| HCl | A (r-LSN) | U (r-LSN) | A (r-LSN) | (63) | |||
| IS | A (m-PN) | AS (m-PN) | AS (m-PN) | A (m-PN) | (17,49) | ||
| AS (m-LSN,PN) | (32) | ||||||
| NaCl (308 mM) | A (r-LSN) | U (r-LSN) | A (r-LSN) | (63) | |||
| PBMCs | S (m-PN) | S (m-PN) | S (m-PN) | S (m-PN) | (48) | ||
| SLIGRL | AU (m-LSN) | (13) | |||||
| TRPV1 | U (r-LSN) | U (r-LSN) | A (r-LSN) | (63) | |||
| A↓(m-LSN) | A (m-LSN) | (12) | |||||
| AU (m-PN) | U (m-PN) | ||||||
| A (m-PN) | A (m-PN) | A (m-PN) | A (m-PN) | (49) | |||
| AU (m-LSN, PN) | (32) | ||||||
| TRPA1 | S (m-PN) | S (m-LSN, PN) | (13) | ||||
| TRPV4 | S (m-PN) | S (m-LSN) | (16) | ||||
| TRPM8 | A↓(m-LSN) | A↓(m-LSN) | (43) | ||||
| IBS/IBD models | |||||||
| DSS | U (r-LSN) | U (r-LSN) | (21) | ||||
| TNBS | S (m-PN) | U (m-PN) | U (m-PN) | S (m-LSN, PN) | S (m-LSN) | (47) | |
| S (m-PN) | ↓(m-PN) | U (m-PN) | S (m-PN) | (31) | |||
| Zymosan | U (m-PN) | U (m-PN) | S (m-PN) | U (m-PN) | S (m-PN) | (33) | |
A, activated; S, sensitized; ↓ mechanical response reduced; U, unaffected; AU, activated but not sensitized; A↓, activated, response decreased; r, rat; m, mouse; PN, pelvic nerve; LSN, lumbar splanchnic nerve; 48/80, compound 48/80 that promotes mast cell degranulation; AHS, acidified hypertonic solution; BK, bradykinin; IS, inflammatory soup; TRP, transient receptor potential cation channel; TRPV1, TRP, subfamily V, member 1; TRPV4, member 4; TRPA1, subfamily A, member 1; TRPM8, subfamily M, member 8; DSS, dextran sodium sulfate; TNBS, 2,4,6-trinitrobenzenesulfonic acid; SLIGRL, protease- activated receptor-2 activating peptide; PBMCs, mediators from peripheral blood mononuclear cells.