Fig. 5.

ASBT level on the plasma membrane is decreased by EPEC infection. A: 2BT cells were infected with wild-type EPEC and then plasma membrane proteins in 2BT cells were labeled by cell surface biotinylation and then pulled down by streptavidin as described in materials and methods. Surface protein (biotinylated) and total cellular proteins were separated by electrophoresis, and ASBT-V5 fusion protein was then detected by Western blot analysis using anti-V5 antibodies. B: 2BT cells were infected for 60 min with EPEC in the absence or the presence of different concentrations (2.5 and 5 μM) of the inhibitor of endocytosis, phenylarsine oxide (PAO). ASBT activity was then assessed, and the data are expressed as % control and represent means ± SE of 3 values from separate occasions. *P < 0.05 compared with control.