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. 2012 Mar 9;302(10):H2122–H2130. doi: 10.1152/ajpheart.00775.2011

Fig. 3.

Fig. 3.

O-GlcNAcylation is essential for calcium-activated NFATc3 activation and nuclear translocation. A: NRCMs expressing NFATc3-GFP protein were co-infected with Ad-OGA or Ad-null in the presence or absence of the calcium. The HBP inhibitor DON was given to the cells 24 h before treatment with calcium. Ad-null was added to samples not containing any adenovirus treatment to serve as a control. DAPI was used to mark the nucleus. B: quantification data of NFATc3-GFP translocation. C: the effect of phenylephrine on Ad-NFAT uciferase reporter activity. Tubulin served as a loading control. The Ad-null was added to samples not containing any adenovirus treatment to serve as a control. Results were normalized to beta-galactosidase activity. Values are expressed as means ± SE, n ≥ 3 per group. **Significant difference (P < 0.01).