Fig. 8.
Effects of sTNFR:Fc on cultured cardiomyocytes after H/R. Cultured cardiomyocytes were exposed to H/R in the presence of vehicle or sTNFR:Fc (5 μg/ml). A: TUNEL assay in cultured cardiomyocytes. The TUNEL-positive nuclei were counted in 6 fields of 5 separate chambers on glass slides at ×400 magnification and are presented as a percentage of the total number of cardiomyocytes (nuclei). B: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The reduction in MTT activity in the treated cells is presented as a percentage of nontreated control cells. C: extracellular release of arachidonic acid from cultured cardiomyocytes. Cardiomyocytes prelabeled with 0.5 μCi/m [3H]arachidonic acid were exposed to H/R in the presence of vehicle or sTNFR:Fc. The radioactivity in the supernatant was measured in a scintillation spectrometer. The activity was expressed as the percentage of total cell radioactivity. *P < 0.01 and †P < 0.01 compared with the respective nontreated control cells (Control); n = 6∼8 mice in each experiment.