Table 1.
Forward Primers | Reverse Primers | |
---|---|---|
sPLA2-IIC | 5′-CCGGGATCCTAGAAAACACA-3′ | 5′-TGTCCCGAACATCCTCTTTC-3′ |
sPLA2-IID | 5′-GAACCACCGGCCTAATTACA-3′ | 5′-GATGAAGGTAGGCTGGGTCA-3′ |
sPLA2-IIE | 5′-CCTGCGAGTGTGACAAGAGA-3′ | 5′-ATGAGTCTGCTGGGAGAGGA-3′ |
sPLA2-IIF | 5′-AACACTCCACTGGACGGAAG-3′ | 5′-GTAGCCCACAAAGGACAGGA-3′ |
sPLA2-III | 5′-GGCTGAGGCCACCTCATATACTTC-3′ | 5′-TCCTTTGCCCTCAGCACAGTCAAG-3′ |
sPLA2-V | 5′-GATGCACGACCGTTGTTATGG-3′ | 5′-AGGAGTCGTGTTCGCAGATGA-3′ |
sPLA2-X | 5′-TTCAGCGAAGCAACCAGGA-3′ | 5′-CACCAAGGCCACAATAACAGC-3′ |
cPLA2α | 5′-CTGGCCAACATCAACTTCAG-3′ | 5′-GCCAGTCTCTTCTGCATAGT-3′ |
cPLA2β | 5′-GTGATTGCCGTGATGGCCACTG-3′ | 5′-CCGGCCAGCTGCCCATACAAAG-3′ |
cPLA2γ | 5′-GGACCAGTGACATACTCAGAAG-3′ | 5′-ACTAAATCCAAGAGCATTGC-3′ |
iPLA2 | 5′-TCAGGATCTCATGCCCATCTCT-3′ | 5′-TGGTCGTGACTCCGCTTCTC-3′ |
GAPDH | 5′-TGCACCACCAACTGCTTAG-3′ | 5′-GATGCAGGGATGATGTTC-3′ |
sPLA2, secretory phospholipase A2 (PLA2); cPLA2, cytosolic PLA2; iPLA2, Ca2+-independent PLA2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.