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. 2012 Mar 16;302(10):H2018–H2030. doi: 10.1152/ajpheart.00955.2011

Table 1.

Sequences of PCR primers

Forward Primers Reverse Primers
sPLA2-IIC 5′-CCGGGATCCTAGAAAACACA-3′ 5′-TGTCCCGAACATCCTCTTTC-3′
sPLA2-IID 5′-GAACCACCGGCCTAATTACA-3′ 5′-GATGAAGGTAGGCTGGGTCA-3′
sPLA2-IIE 5′-CCTGCGAGTGTGACAAGAGA-3′ 5′-ATGAGTCTGCTGGGAGAGGA-3′
sPLA2-IIF 5′-AACACTCCACTGGACGGAAG-3′ 5′-GTAGCCCACAAAGGACAGGA-3′
sPLA2-III 5′-GGCTGAGGCCACCTCATATACTTC-3′ 5′-TCCTTTGCCCTCAGCACAGTCAAG-3′
sPLA2-V 5′-GATGCACGACCGTTGTTATGG-3′ 5′-AGGAGTCGTGTTCGCAGATGA-3′
sPLA2-X 5′-TTCAGCGAAGCAACCAGGA-3′ 5′-CACCAAGGCCACAATAACAGC-3′
cPLA2α 5′-CTGGCCAACATCAACTTCAG-3′ 5′-GCCAGTCTCTTCTGCATAGT-3′
cPLA2β 5′-GTGATTGCCGTGATGGCCACTG-3′ 5′-CCGGCCAGCTGCCCATACAAAG-3′
cPLA2γ 5′-GGACCAGTGACATACTCAGAAG-3′ 5′-ACTAAATCCAAGAGCATTGC-3′
iPLA2 5′-TCAGGATCTCATGCCCATCTCT-3′ 5′-TGGTCGTGACTCCGCTTCTC-3′
GAPDH 5′-TGCACCACCAACTGCTTAG-3′ 5′-GATGCAGGGATGATGTTC-3′

sPLA2, secretory phospholipase A2 (PLA2); cPLA2, cytosolic PLA2; iPLA2, Ca2+-independent PLA2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.