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. 2012 Feb 29;302(9):R1034–R1048. doi: 10.1152/ajpregu.00596.2011

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Fig. 1. — Native PAGE activity assay characterization. A: blue native (BN)-PAGE Complex IV activity assay characterization. B: BN-PAGE Complex V activity assay characterization. C: clear native (CN)-PAGE Complex IV activity assay characterization. D: CN-PAGE Complex V activity assay characterization. For A–D: 1) Coomassie-stained native gel; 2) gel activity assay at 10, 20, 40, and 60 min; 3) a plot of activity relative to protein concentration; and 4) a plot of activity as a function of time for the 25-, 50-, and 75-μg lanes. E: CN-PAGE Complex V activity assay characterization with ethanol control (E1)or 50 μM oligomycin (E2). Left, paired Coomassie-stained CN-PAGE gel; right, Complex V activity data at 10, 20, 40, and 60 min.