Fig. 3.

Stimulation of phagocytic NADPH oxidase by PMA in the presence of mitochondrial inhibitors. Superoxide production was measured in unstimulated or PMA-stimulated lymphoblasts in the presence of ethanol as a vehicle, inhibitor of mitochondrial complex I rotenone (10 μM), mitochondrial proton ionophore CCCP (10 μM), inhibitors of mitochondrial complex III stigmatellin (10 μM), or antimycin A (10 μM). Rotenone significantly increased activity of phagocytic NADPH oxidase similar to CCCP, while inhibitors of complex III stigmatellin and antimycin A further increased O₂⁻ production compared with PMA + CCCP-treated cells. Results are expressed as means ± SE; n = 3–6. *P < 0.001 vs. Control. **P < 0.05 vs. PMA. ***P < 0.01 vs. PMA. +P < 0.01 vs. PMA+CCCP. #P <0.001 vs. PMA + rotenone.