Fig. 1.

Effects of IL-6 on endothelial permeability and inflammatory signaling. A: at the time indicated by arrow, pulmonary endothelial cell (EC) plated on microelectrodes were treated with either vehicle or a mixture of IL-6 and its soluble receptor (SR; 15 ng/ml/3.75 ng/ml; 30 ng/ml/7.5 ng/ml; 40 ng/ml/10 ng/ml; 60 ng/ml/15 ng/ml; 100 ng/ml/25 ng/ml) and used for measurements of transendothelial electrical resistance (TER). B: cells were treated with IL-6 and SR (40 ng/ml/10 ng/ml) for indicated periods of time. Levels of phosphorylated proteins or total IκBα content in the cell lysates were determined by Western blot analysis using specific antibodies. Equal protein loading was confirmed by determination of β-tubulin content in total cell lysates. Rearranged lanes from the same blot are outlined by vertical dotted line. Shown are representative results of 3–5 independent experiments. Stat, signal transducers and activators of transcription; HSP27, heat shock protein 27.