Fig. 11.
Effect of treatment with NH4Cl on pulmonary arteriolar remodeling in hypoxic and MCT models of PH. A: representative hematoxylin and eosin-stained lung sections from control normoxic, hypoxic (2 wk), and MCT-treated rats with or without treatment with NH4Cl (acidosis). Pulmonary arterioles 50–100 μm in diameter are indicated with arrows. Total magnification ×20. Scale bar = 50 μm. B: quantitative morphometric analysis of % wall thickness of pulmonary arterioles defined as the area occupied by the vessel wall divided by the total cross-sectional area of the arteriole. Fifteen pulmonary arterioles (50–100 μM diameter) from 5 rats per experimental group were analyzed by 2 independent investigators. Percent wall thickness was measured and presented as means ± SE. *Measurements in hypoxic or MCT-treated rats are significantly different (P < 0.05) from corresponding measurements in control normoxic or vehicle-treated rats. #Measurements in NH4Cl-treated hypoxic or MCT-treated rats are significantly different (P < 0.05) from corresponding measurements in hypoxic or MCT-treated rats without NH4Cl treatment.