Table 2.
Phe contraction, and ACh and SNP relaxation in pulmonary and mesenteric arteries of control normoxic, hypoxic and MCT-treated rats with or without treatment with NH4Cl (acidosis)
| Parameter | Normoxia | Normoxia+Acidosis | Hypoxia | Hypoxia+Acidosis | MCT | MCT+Acidosis |
|---|---|---|---|---|---|---|
| Pulmonary artery | ||||||
| Phe Max, 10−5 M contraction, g/mg | 0.88 ± 0.08 | 0.89 ± 0.12 | 0.24 ± 0.03* | 0.43 ± 0.07*† | 0.30 ± 0.07* | 0.63 ± 0.16† |
| +l-NAME, 3×10−4 M | 1.02 ± 0.15 | 0.88 ± 0.17 | 0.37 ± 0.09* | 0.31 ± 0.05* | 0.33 ± 0.11* | 0.66 ± 0.22† |
| +ODQ, 10−5 M | 1.11 ± 0.17 | 1.33 ± 0.30 | 0.34 ± 0.06* | 0.35 ± 0.06* | 0.45 ± 0.11* | 0.73 ± 0.23 |
| Phe pED50, −log M | 7.71 ± 0.08 | 7.68 ± 0.09 | 7.67 ± 0.09 | 7.46 ± 0.09 | 7.58 ± 0.16 | 7.53 ± 0.08 |
| +l-NAME | 7.56 ± 0.13 | 7.82 ± 0.13 | 7.47 ± 0.17 | 7.32 ± 0.14 | 7.20 ± 0.18 | 7.42 ± 0.11 |
| +ODQ | 7.81 ± 0.10 | 7.66 ± 0.11 | 7.67 ± 0.14 | 7.59 ± 0.15 | 7.76 ± 0.10 | 7.61 ± 0.15 |
| Phe contraction % 96 mM KCl | 94.97 ± 2.23 | 96.46 ± 4.09 | 94.04 ± 5.90 | 93.64 ± 5.10 | 113.51 ± 10.23 | 104.96 ± 5.53 |
| +l-NAME | 106.17 ± 4.67‡ | 113.93 ± 11.72 | 185.65 ± 66.34 | 113.51 ± 11.15 | 158.80 ± 19.57*‡ | 142.65 ± 11.66‡ |
| +ODQ | 116.72 ± 4.53‡ | 114.25 ± 7.18‡ | 133.74 ± 13.06‡ | 135.85 ± 15.50‡ | 194.34 ± 24.66*‡ | 155.33 ± 10.28*‡ |
| ACh, 10−5 M % relaxation | 48.56 ± 2.88 | 45.33 ± 4.81 | 14.35 ± 5.14* | 31.92 ± 5.53*† | 7.98 ± 3.00* | 18.54 ± 2.42*† |
| SNP, 10−5 M % relaxation | 91.86 ± 2.78 | 95.30 ± 1.56 | 48.18 ± 10.72* | 78.28 ± 6.21† | 58.45 ± 6.07* | 82.22 ± 4.70† |
| Mesenteric artery | ||||||
| Phe Max, 10−5 M contraction, g | 1.26 ± 0.14 | 0.93 ± 0.07 | 0.97 ± 0.12 | 1.06 ± 0.16 | 1.08 ± 0.16 | 1.00 ± 0.14 |
| pED50, −log M | 6.28 ± 0.12 | 6.57 ± 0.15 | 6.15 ± 0.12 | 6.26 ± 0.09 | 6.63 ± 0.25 | 6.55 ± 0.16 |
| ACh, 10−5 M % relaxation | 93.26 ± 3.19 | 77.43 ± 12.21 | 94.18 ± 4.10 14 | 88.68 ± 7.35 12 | 87.45 ± 6.68 | 95.53 ± 2.74 |
| SNP, 10−5 M % relaxation | 96.20 ± 2.02 | 98.85 ± 1.15 | 92.86 ± 7.14 | 100.0 ± 0.00 | 90.48 ± 7.14 | 97.74 ± 1.45 |
Data represent means ± SE of cumulative data from 6–8 rats.
Measurements in hypoxia or MCT-treated rats are significantly different (P < 0.05) from corresponding measurements in normoxic rats.
Measurements in NH4Cl-treated (acidosis) rats are significantly different (P < 0.05) from corresponding measurements in normoxic, hypoxic, or MCT rats without NH4Cl treatment.
Measurements in Nω-nitro-l-arginine methyl ester (l-NAME) or guanylate cyclase inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ)-treated arteries are significantly different from corresponding measurement in nontreated arteries. Phe, phenylephrine; Max, maximum; SNP, sodium nitroprusside.