Fig. 3.

BQ610 increases TGF-β₂ and -β₃ mRNA and pSmad2 protein by Western blot in lung homogenates at 14 days of age. A–C: mRNA levels of TGF-β₁ (A), TGF-β₂ (B), and TGF-β₃ (C) measured by real-time quantitative RT-PCR (qPCR) in homogenized lungs from mice exposed to air or hypoxia in combination with either vehicle or BQ610 from birth to 14 days. D: representative Western blot of lung homogenates for pSmad2 and β-tubulin from lung homogenates of mouse pups given either vehicle or BQ610 during 14 days of air or hypoxia exposure. E: pSmad2/β-tubulin ratio in Western blots quantitated by densitometry (n = 4–6 mice/group; data are means ± SE; *P < 0.05 vs. corresponding air at same time point; #P < 0.05 vs. corresponding vehicle).