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. 2012 Jan 27;302(9):L857–L865. doi: 10.1152/ajplung.00258.2011

Fig. 4.

Fig. 4.

Inhibition of TGF-β signaling reduces endothelin-1 (ET-1) immunohistochemical staining and ET-1 synthesis in vivo. A–D: representative photomicrographs of lung sections stained for ET-1 from mouse pups given either vehicle (A and B) or 1D11 (TGF-β neutralizing antibody; C and D) during 14 days of air (A and C) or hypoxia (B and D) exposure (PA, pulmonary artery; Br, bronchiole; ×400; calibration bars = 50 μm). ET-1 (brown staining), primarily in pulmonary artery endothelium, is increased in hypoxia-vehicle mice and reduced in hypoxia-1D11 mice. Homogenized lungs from mouse pups exposed to air or hypoxia in combination with either vehicle or 1D11 from birth to 14 days were used for measurement of mRNA levels of ET-1 by real-time qPCR (E) and ET-1 protein by ELISA (G). F: mRNA levels of ET-1 were measured by qPCR in DNTGFβRII mouse pups exposed to air or hypoxia from birth to 14 days of age in combination with either vehicle or zinc (inducer of DNTGFβRII) (n = 6 mice/group; data are means ± SE; *P < 0.05 vs. corresponding air at same time point; #P < 0.05 vs. corresponding vehicle).