Fig. 1.

CC chemokine ligand-2 (CCL2) protein was upregulated after bleomycin-induced lung injury. A: 8- to 10-wk old C57Bl/6 wild-type (WT) mice and Ccl2 transgenic mice were treated with bleomycin at 5 U/kg intratracheally. Mice were killed at indicated time points and mouse CCL2 proteins in bronchoalveolar lavage (BAL) were measured using ELISA (n = 4–5 mice; *P < 0.05, ***P < 0.001). B: high levels of human CCL2 were expressed in Ccl2 transgenic mouse BAL with and without bleomycin challenge (n = 3–5 mice).