Fig. 4.

Stimulation of PKC results in increased UT-A1 phosphorylation. Metabolically labeled (³²P) rat IMCDs were incubated without (control, Ctrl) or with 2 μM phorbol dibutyrate (PDBu) for 30 min, and then UT-A1 was immunoprecipitated and analyzed by Western blot and autoradiography. A: autoradiogram of ³²P-UT-A1. B: Western blot of total UT-A1 for representative samples. Arrows indicate the 117- and 97-kDa glycoprotein forms of UT-A1. C: bar of the ratio of phosphorylated UT-A1 to total UT-A1 from all samples presented as means ± SE, n = 6/condition. *P < 0.05 vs. Ctrl.